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antigen gapdh  (Santa Cruz Biotechnology)


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    Santa Cruz Biotechnology antigen gapdh
    Antigen Gapdh, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/antigen+gapdh/pmc10758042__jcav15p0370s1-15-0-22?v=Santa+Cruz+Biotechnology
    Average 90 stars, based on 1 article reviews
    antigen gapdh - by Bioz Stars, 2026-07
    90/100 stars

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    Real-time PCR primer synthesis list

    Journal: Open Medicine

    Article Title: EphA3 targeted by miR-3666 contributes to melanoma malignancy via activating ERK1/2 and p38 MAPK pathways

    doi: 10.1515/med-2022-0597

    Figure Lengend Snippet: Real-time PCR primer synthesis list

    Article Snippet: Proteins (20 μg per lane) were resolved in 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis and detected on poly(vinylidene fluoride) membranes after which was blocked by 5% skim milk powder at room temperature for 2 h. Protein detection on membranes was detected with anti-p38MAPK (FNab06077, 1:1,000, FineTest, Biological Technology Co., Ltd, China), anti-p-p38MAPK (44-684G, 1:1,000, ThermoFisher), anti-p-ERK1/2 (44-680G, 1:1,000, ThermoFisher), anti-ERK1/2 (13-6200, 1:1,000,ThermoFisher), anti-EphA3 (310438-T08, 1:1,000, SinoBiological, China), and anti-GAPDH (100242-T08, 1:1,000, SinoBiological) at 4°C for 24 h. Next, horseradish peroxidase-conjugated secondary antibodies (A32731, 1:1,000, ThermoFisher) were added and incubated with these membranes at room temperature for 1 h. Immune blots were developed with enhanced chemiluminescence (GE Healthcare, USA) and counted quantitatively by ImageJ.

    Techniques: Real-time Polymerase Chain Reaction